{"id":3536,"date":"2018-08-27T07:52:06","date_gmt":"2018-08-27T07:52:06","guid":{"rendered":"http:\/\/www.megaflora9.com\/wp\/?p=3536"},"modified":"2018-09-26T05:59:30","modified_gmt":"2018-09-26T05:59:30","slug":"action-at-level-2-influence-on-the-epithelial-barrier-function","status":"publish","type":"post","link":"https:\/\/megaflora9.com\/?p=3536","title":{"rendered":"Action at Level 2: influence on the epithelial barrier function"},"content":{"rendered":"

Maintaining intestinal homeostasis requires that the epithelium,\u00a0a layer of cells lining the intestinal wall, keeps\u00a0formeing an uninterrupted barrier without spaces or\u00a0gaps between the enterocytes. This optimal situation is\u00a0known as barrier effect<\/strong>.<\/p>\n

Normally, in a healthy person there is a communication\u00a0and control between the host and microbiota residing in\u00a0the gut, which creates a homeostatic balance of bacterial\u00a0flora by promoting the growth of beneficial bacteria, preventing\u00a0overgrowth and controlling the development of\u00a0pathogenic bacteria.\"\"<\/p>\n

The phenomena of dysbiosis, or imbalance of intestinal\u00a0flora, invariably lead to bacterial overgrowth of the\u00a0small intestine called SIBO (Small Intestine Bacterial\u00a0Overgrowth), which tilts the balance toward the growth\u00a0of pathogenic bacteria.<\/p>\n

It is also known that situations such as food intolerances,\u00a0presence of allergens like gluten, digestive disorders\u00a0and even lifestyle are factors that increase intestinal permeability,\u00a0losing the integrity of the intestinal mucosa.<\/p>\n

All these unfavourable conditions taken together lead to\u00a0an imbalance in the intestine that causes an alteration\u00a0in the absorption processes, with the consequent lack of\u00a0nutrients and micronutrients in the body, essential for a\u00a0healthy live and, in the more severe states, favours the\u00a0passage of toxic substances into the body.<\/p>\n

The damage caused by the intestinal imbalance not only\u00a0cause situations such as irritable bowel syndrome or\u00a0Crohn\u2019s disease, but some scientific studies claim that\u00a0high permeability of the intestine can be the cause of\u00a0many mental and nervous illnesses, such as senile dementia,\u00a0Alzheimer or Parkinson among others.<\/p>\n

<\/div>\n

Megaflora 9 evo<\/strong> has demonstrated its ability to counter\u00a0the phenomena of dysbiosis rebalancing the intestinal\u00a0flora, maintaining the barrier effect and promoting the integrity\u00a0of the gut mucosa, that allows the maintenance of\u00a0the absorption processes and homeostasis between the\u00a0host and the intestinal microbiota.<\/p>\n

The integrity of the intestinal epithelium can be measured\u00a0by an in vitro test that measures the Trans-Epithelial\u00a0Electrical Resistance (TEER).<\/p>\n

TEER methodology<\/h2>\n

TEER is a widely accepted quantitative technique that\u00a0measures the dynamic integrity of cell junctions (tight\u00a0junction) in models of endothelial and epithelial monolayers.\u00a0TEER values are reliable indicators of the integrity\u00a0of cell barriers for assessing the transport of drugs and\u00a0other substances.<\/p>\n

The values obtained in the TEER inform in real time reports\u00a0about the integrity or alteration of monolayer cells, which\u00a0is manifested by the electrical variation of values between\u00a0the upper and the lower chambers. When the monolayer\u00a0is subjected to the presence of a stressor in the apical\u00a0part (upper chamber), the change in permeability can be\u00a0measured as a decrease TEER between the upper and lower\u00a0chamber.<\/p>\n

The effectiveness of a probiotic strain on maintaining the\u00a0barrier effect while placing the stressor can be detected\u00a0by smaller decreases in TEER. The lesser decrease in TEER,\u00a0the more effectiveness of the probiotic in maintaining the\u00a0integrity of the monolayer and therefore it will preven the\u00a0increase of permeability in presence of stressor agents.<\/p>\n

\"\"

TEER Methodology<\/p><\/div>\n

To determine the barrier effect of Megaflora 9 evo<\/strong>, a\u00a0Caco-2 monolayer cells was subjected to the presence of\u00a0a stressor which increases the monolayer permeability.<\/p>\n

The result showed that Megaflora 9 evo<\/strong> had a positive\u00a0effect on the integrity of the monolayer cells, reducing\u00a0the negative impact of the stressor compared\u00a0to control.\"\"<\/p>\n

Change in TEER in a monolayer of Caco-2 cells subjected to the presence of a stressor (red) and in\u00a0the presence of the stressor and Megaflora 9 evo<\/strong> (green), relative to the control without stressor (blue)<\/p>\n

Upon this result, it follows that the composition of Megaflora\u00a09 evo<\/strong> has a balancing effect on the microflora\u00a0in the intestine, avoiding the increased permeability of\u00a0the intestinal mucosa caused by the presence of certain\u00a0substances, pathogens or situations that influence the\u00a0regulation of the bowel activity.<\/p>\n

>> Action at Level 3: immunomodulation<\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"

Maintaining intestinal homeostasis requires that the epithelium,\u00a0a layer of cells lining the intestinal wall, keeps\u00a0formeing an uninterrupted barrier wit<\/span><\/div>\n
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